Compatibility summary based on MALDIID Final Report

B-C95-P01B01 (04 September 2025)

Executive Summary

Short-term (2–6 hours): GLP shows partial compatibility in the tank with Roundup Ready granules; total viable counts retain approximately 64–68% of initial levels.

Overnight (24 hours): Total viable counts drop to ~18% of the starting level, driven by marked declines in Lactobacillus groups. The mix should be sprayed within the same day and preferably within 2–6 hours of mixing.

What Was Tested

Sample 1: Roundup Ready with PlantShield granules (1500 g/ha) + GLP (batch C25.024) at 4 L/ha; counts at 0 h, 2 h, 6 h, and 24 h.

Sample 2: GLP at 4 L/ha baseline counts at 0 h (no time-course).

Overall Viability Trend

Total viable counts (“Total all”) over time in the combined mix:

Detailed Results

Colony-forming units (CFU/mL) and retention relative to 0 h for Roundup Ready + GLP mix:

Microbe group Control 0h (CFU/mL) 0 h (CFU/mL) 2 h (CFU/mL) 6 h (CFU/mL) 24 h (CFU/mL) Retention @2 h (%)

Retention @24 h (%)
Candida 1.00E+05 2.00e+05 3.00e+05 9.00e+05 2.20e+06 150.0 1100.0
Acetobacter 3.00E+05 1.00e+05 2.00e+05 8.00e+04 5.00e+05 200.0 500.0
L. buchneri 1.70E+07 1.60e+07 2.00e+07 2.00e+07 1.00e+06 125.0 6.2
L. casei 3.60E+07 5.00e+07 2.20e+07 1.50e+07 8.00e+06 44.0 16.0
L. rapi/paracasei 8.00E+06 2.00e+06 4.00e+06 8.00e+06 5.00e+05 200.0 25.0
Total Lactobacillus 6.10E+07 6.80e+07 4.60e+07 4.30e+07 9.50e+06 67.6 14.0
Total all 6.14E+07 6.83e+07 4.65e+07 4.40e+07 1.22e+07 68.1 17.9

Interpretation

  • Compatible for short tank times: If mixed and sprayed within a few hours (2–6 h), most viable counts remain (≈64–68% retained), indicating partial compatibility.
  • Not stable if held overnight: By 24 h, overall viable counts drop ~82%, primarily due to declines in Lactobacillus (buchneri, casei, paracasei). Avoid holding the mix overnight.
  • Microbial balance shifts: Yeasts (Candida) and Acetobacter increase, while Lactobacillus groups decrease. If outcomes rely on Lactobacillus activity, time-to-spray is critical.

Recommendations

  1. Spray window: Mix and spray within 2–6 hours; do not hold overnight.
  2. Order of addition: Add GLP last into the tank after water, conditioners, and herbicide; maintain gentle agitation and avoid foaming.
  3. Water quality: Use clean, low-chlorine water and avoid extreme pH below 4 or high salinity.
  4. Operational approach: Make smaller tanks to minimize hold time if logistics require extended spraying.
  5. Label compliance: Follow Roundup Ready and GLP labels and Terragen guidance.

Reference: MALDIID Pty Ltd, Final Report – B-C95-P01B01, dated 04 September 2025